Synergistic activation of transcription by cbp and p53

Activation of cAMP and mitogen responsive genes relies on a common nuclear factor. A family of transcriptional adaptor proteins targeted by the E1A oncoprotein.

Adenoviral E1A-associated protein p as a functional homologue of the transcriptional co-activator CBP. Show 10 more references 10 of Smart citations by scite. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles. Explore citation contexts and check if this article has been supported or disputed.

Regulation of p53 by E3s. Human papillomavirus E6 and E7: What remains? RBM10, a New Regulator of p Data Data that cites the article This data has been provided by curated databases and other sources that have cited the article. QuickGO Annotations. Protein Interactions 5. Similar Articles To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.

The transcriptional co-activator proteins p and CBP stimulate adenovirus E1A conserved region 1 transactivation independent of a direct interaction. Synergistic role of E1A-binding proteins and tissue-specific transcription factors in differentiation.

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Annotations API. OAI service. Bulk downloads. Developers Forum. Here we demonstrate that the transcription factor CBP, which is also implicated in cell proliferation and differentiation, acts as a p53 coactivator and potentiates its transcriptional activity. The amino-terminal activation domain of p53 interacts with the carboxy-terminal portion of the CBP protein both in vitro and in vivo. In transfected SaoS-2 cells, CBP potentiates activation of the mdm-2 gene by p53 and, reciprocally, p53 potentiates activation of a Gal4-responsive target gene by a Gal4 -CBP fusion protein.

As sequence-specific transcriptional activation by p53 correlates with its ability to suppress cell growth 1 , 2 , 3 , cell-growth suppression by CBP may be mediated, at least in part, though synergistic activation with p53 on its target genes. CBP is also a target of adenovirus E1a 5 — 14 and this work and our results indicate that the binding of E1a to CBP could suppress the synergistic effect of CBP and p53 on transcriptional activation, which could also be essential for the transformation activity of this oncoprotein.

A number of activators that interact with CBP 4 — 14 , and this work interact both physically and functionally with other coactivators: these coactivators include specific TAFs in the case of p53 refs 17 , 18 and CREB ref. The fact that p53 exists as a tetramer in vivo should allow it to interact simultaneously with CBP and other coactivators TAFs.

This may be essential for optimal activation in vivo and also explains the synergy between CBP and p53, even when CBP is tethered to the promoter by an alternative mechanism. Plasmids and fusion proteins. GST fusion proteins were expressed in E. Plasmids encoding p53 wild-type or mutant proteins were created by amplification of the appropriate DNA fragments, including the Flag sequence, followed by subcloning into pETd Novagene.

Affinity chromatography. In vitro binding assays. Beads were then washed six times in 1 ml BC0 buffer containing KCl and 0. Coimmunoprecipitation assay. Transient transfection assays. All transfections were done in duplicate; representative experiments depict the average of three experiments with standard deviations indicated. After incubation with the indicated antibodies for 1 h at room temperature, blots were subsequently incubated with secondary antibodies and visualized by ECL as suggested by the manufacturer Amersham.

Ko, L. Genes Dev. El-Deiry, W. WAF1, a potential mediator of p53 tumor suppression. Cell 75 , — Wu, X. The pmdm-2 autoregulatory feedback loop. Chrivia, J. Nature , — Yang, X. Kwok, R. Arias, J. Activation of cAMP and mitogen responsive gene relies on a common nuclear factor. Arany, Z. Afamily of transcriptional adaptor proteins targeted by the E1a oncoprotein. Nature , 81—84 Lundblad, J. Adenoviral E1a-associated protein p as a functional homologue of the transcriptional coactivator CBP.

Nature , 85—88 Banister, A. CBP-induced stimulation of c-Fos activity is abrogated by E1a. EMBO J. Article Google Scholar. Dai, P. CBP as a transcriptional coactivator of c-Myb. Kamei, Y. ACBP integrator complex mediates transcriptional activation and AP-1 inhibition by nuclear receptors. Cell 85 , — Chakravati, D. Nature , 99— Lee, J. Adenovirus E1a downregulates c-Jun and JunB-mediated transcription by targeting their coactivator p Ogryzko, V.

The transcriptional coactivator p and CBP are histone acetyltransferase. Cell 87 , — Bannister, A. The CBP coactivator is a histone acetyltransferase. Thut, C. Science , — Lu, H. Human TAF31 protein is a transcriptional coactivator of the p53 protein.

Natl Acad. USA 92 , — Xiao, H. Seto, E.